Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Patent
1989-08-28
1992-12-08
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
43525233, C12P 1322, C12N 121
Patent
active
051697688
ABSTRACT:
A method of controlling of cell mass by starving cells that are ppc.sup.- and pc.sup.- for tricarboxylic acid cycle (TAC) intermediates, while fermenting a product compound. The cells are adapted to enable or enhance conversion of a raw material to the product via a bioconversion pathway that does not include any TAC intermediates, and preferably they are either conditionally or permanently incapable of producing a net increase in TAC intermediates via any anaplerotic pathways. First the cells are cultured to the desired cell mass under growth medium conditions characterized by the presence of at least one TAC intermediate, or by the presence of a compound that the cells can convert to a TAC intermediate via an anaplerotic pathway under the conditions present in the growth medium. In that way the medium provides the cell enough TAC intermediates to support growth. When the desired cell density has been reached, the cells are cultured under product-production medium conditions in which the cell does not receive adequate TAC intermediates to support growth, because the medium lacks TAC intermediates as well as compounds that they all can convert to TAC intermediate via an anapleortic pathway.
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BioTechnica International, Inc.
H.J. Heinz Company
Schwartz Richard A.
Vogel Nancy T.
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