Method of binding enzymes to sintered expanded clays

Details Method of binding enzymes to sintered expanded clays Method of binding enzymes to sintered expanded clays

1992-12-10

1995-08-22

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Multi-enzyme system

435176, 435180, 435181, 435182, 4352625, C12N 1118, C12N 1114, C12N 1108, C12N 1104

Patent

active

054439752

ABSTRACT:
Enzymes, cells and/or cellular organelles are bound to an insoluble sintered expanded clay support matrix for catalyzing transformations of agriculture and industrial residues in soil and in other environments. In one embodiment, an enzyme is absorbed by the sintered expanded clay support matrix and a phenolic monomer is polymerized or copolymerized on the support matrix containing the bound enzyme. In another embodiment, an enzyme different from the enzyme absorbed by the support matrix is combined with the phenolic monomer and a copolymer of enzyme and phenolic monomer is formed on the support matrix containing the bound enzyme. The phenolic monomer is preferably catechol, pyrogallic acid and/or resorcinol. Polymerization or copolymerization of the phenolic monomer is catalyzed by enzyme catalysis with laccase or tyrosinase or peroxidase in combination with hydrogen peroxide, by inorganic catalysis with ammonium persulfate or manganese dioxide, or by organic catalysis with N,N,N',N' tetramethyl-ethylene-diamine (TEMED).

REFERENCES:
patent: 2717852 (1955-09-01), Stone
patent: 3556945 (1971-01-01), Messing
patent: 3933589 (1976-01-01), Keyes
patent: 3982997 (1976-09-01), Eaton et al.
patent: 4226938 (1980-10-01), Yoshida et al.
patent: 4585738 (1986-04-01), Roland

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