Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-11-04
1994-02-15
Griffin, Ronald W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436503, 424 70, 424450, 514844, 435 72, A61K 9127, A61K 4748
Patent
active
052866297
DESCRIPTION:
BRIEF SUMMARY
The present invention relates essentially to a method of binding a product to the membrane of a keratinocyte by means of a ligand-receptor bond, to a method of preparing such a product, to the product obtained, to a cosmetic or pharmaceutical composition in which it is present and to its method of preparation.
The existence of processes for the recognition of cells, for example in relation to other cells or substances such as proteins, has been known for a long time. These processes act by way of a reaction between a ligand and a receptor present on the surface of the cells.
For example, the recognition of surface antigens by monoclonal antibodies is known, this phenomenon being utilized in therapeutic or diagnostic applications. There may be cited, for example, French patent document A1-2 606 034, which describes antibodies capable of recognizing a new surface protein and of being used especially as reagents in techniques based on the antigen-antibody reaction.
Recognition processes are also known which involve sugars. These are recognized specifically by membrane receptors. However, the sugars recognized are not the same from one type of cell to another. It is known, for example, that rat hepatocytes specifically recognize galactose (Ashwell G. and Morell A. G., Advan. Enzymol. (1974) 41, 99-128) whereas human fibroblasts recognize mannose-6-phosphate.
Based on this acquired knowledge, attempts have been made to develop targeted drug delivery systems making it possible to reach those sites in the organism on which it is desired to act. Liposomes have been used in particular for this purpose.
Since their discovery by BANGHAM (J. Mol. Biol., 13, 238-252 (1965)), liposomes have been used as vehicles for medicinal or other active principles (see, for example, French patent documents A-2 221 122, A-2 415 460 or A-2 540 381).
French patent document A-2 609 397 relates to the use of a substance or composition of carbohydrate type as the active principle of a dermatological, cosmetological, pharmaceutical or cell stimulating composition for the purpose of boosting the cell or tissue bioenergetic potential (see claim 1) or for improving skin comfort, by virtue of their moisturizing, smoothing and softening effect (page 3, line 33 to page 4, line 10, where even an antiinflammatory action is indicated).
Said document therefore concerns a general teaching which does not relate to the particular problem of keratinocytes.
Patent document PCT/WO 87/05300 to BIOCOMPATIBLES LTD relates to a method of preserving a material having a water-dependent structure, which comprises bringing the material into contact with an aqueous solution of a polyhydroxyl compound and then removing the water from this material, the object being to permit the long-term preservation of biological materials such as hemoglobin, erythrocytes, liposomes and cells (see page 1, lines 1 to 4).
Said document concerns the problem of the drying of proteins from aqueous solutions containing saccharides in order to avoid chemical and physical degradation processes (see page 3, 2nd complete paragraph).
More recently, it has been proposed to give liposomes specificity (see BARBET J. et al., "Monoclonal Antibody covalently coupled to liposomes: Specific Targeting to Cells" in J. Supramol. Struct. Cell. Biochem., 16, 243-258 (1981, and LESERMAN L. D. et al., "Targeting to Cells of fluorescent Liposomes covalently coupled with Monoclonal Antibodies or protein A" in Nature, 228, 602-604 (1980)).
Furthermore, patent document WO 88/00474 describes a lipid membrane structure comprising a lipid vesicle encapsulating a drug, and a ligand containing a residue specific for hepatobiliary receptors, for the purpose of directing the structure towards the hepatocytes with an exceptional efficacy.
European patent document A-0 028 917 relates to lipid vesicles carrying hydrocarbon surfaces as lymphatically targeted vehicles for therapeutic and diagnostic purposes. Said document does not concern the targeting of keratinocytes.
European patent document A-0 285 178 relates to ma
REFERENCES:
patent: 3969540 (1976-07-01), Jensen
patent: 4508693 (1990-10-01), Siddiqi et al.
patent: 4826680 (1989-05-01), Jaeger
Chemical Abstracts, vol. 106, No. 23, 106:193579j, Jun. 8, 1987.
Biochimica et Biophysica Acta, vol. 946, 1988, Elsevier Science Publishers B.V., J. Haensler et al.: "Preparation of neo-galactosylated liposomes and their interaction with mouse peritoneal macrophages", pp. 95-105, see especially p. 99, FIG. 1.
Denis Alain
Kieda Claudine
Monsigny Michel
Perrier Pierre
Redziniak Gerard
Griffin Ronald W.
Parfums Christian Dior
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