Method of bilirubin assay

Details Method of bilirubin assay Method of bilirubin assay

1991-05-08

1994-01-11

Housel, James C.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Bilirubin or uric acid standard or control

436 97, 436903, G01N 3100

Patent

active

052780732

ABSTRACT:
In the assaying of direct and indirect bilirubin, it is has been found that organic compounds, capable of undergoing diazotization to products having absorption spectrum substantially identically to bilirubin, can be used as a calibration standard. Typical of these organic compounds is ANS (8-anilino-1-naphthalene-sulfonic acid). However, these compounds do not have the typical yellow color of bilirubin. It was also discovered that this deficiency can be corrected by the addition of methyl or ethyl orange. When combined, these compounds provide a liquid control stable at room temperature for 12 months or more.

REFERENCES:
patent: 4056357 (1977-11-01), Snyder et al.
patent: 4313976 (1982-02-01), Leach
patent: 4612290 (1986-09-01), Yazawa et al.
patent: 4683208 (1987-07-01), Aoyama et al.
patent: 4892833 (1990-01-01), Weiss et al.
Weast C. Robert, "CRC Handbook of Chemistry and Physics", 1982-1983, p. D157.
Aldrich, Catalog Handbook Of Fine Chemicals, 1988 p. 118.

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