Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Patent
1998-02-12
2000-08-08
Smith, Lynette R. F.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
800320, 435468, A01H 100, A01H 500, A01H 4100, C12N 1582, C12N 1587
Patent
active
061004472
ABSTRACT:
A method for stably transforming barley from mature barley seeds as starting material is disclosed. The method involves germinating mature barley seeds until early shoot development occurs, exposing scutellar or embryo tissue cells on the embryo side of germinated seeds, and introducing foreign DNA into the cells. The cells are initially grown under conditions that allow expression of a selectable marker introduced with the foreign DNA, then on a callus-growth medium effective to suppress callus formation in the absence of the selectable marker. Successfully transformed calli can be cultured in suspension to obtain a desired foreign protein, or regenerated into plants, to obtain the foreign protein from the transformed plants, e.g., germinated seeds.
REFERENCES:
Potrykus. Ann. Review of Plant Physiol. 1991. vol. 42: 205-225.
Wan and Lemaux. Plant Physiology. 1994. vol. 104: 37-48.
Chan et al. The Journal of Biological Chemistry. 1994. vol.269: 17635-17641.
Chandler et al. Plant Molecular Biology. 1993. Nov. issue. vol. 23:737-747.
Rodriguez Raymond L.
Wu Liying
Applied Phytologics, Inc.
Dehlinger Peter J.
Petithory Joanne R.
Smith Lynette R. F.
Zaphmont Onsawa M-Foiz
LandOfFree
Method of barley transformation does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of barley transformation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of barley transformation will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1151716