Method of assaying whole blood for HDL cholesterol

Details Method of assaying whole blood for HDL cholesterol Method of assaying whole blood for HDL cholesterol

1992-10-13

1995-10-24

Czaja, Donald E.

Chemistry: analytical and immunological testing

Lipids, triglycerides, cholesterol, or lipoproteins

436169, 436170, 436177, 472 56, 472 57, 435 11, G01N 3392, G01N 118, G01N 2177

Patent

active

054609747

ABSTRACT:
An improved device and method of a) separating the cellular components of whole blood from plasma or serum, b) separating the low density lipoprotein (LDL) fraction and the very low density lipoprotein (VLDL) fraction from the plasma or serum, then c) assaying the plasma or serum for cholesterol present in the high density lipoprotein (HDL) fraction are disclosed. The device includes a separation area that separates the cellular components of whole blood from the serum or plasma and separates the LDL and VLDL fractions from the serum or plasma, and a test area that assays the serum or plasma for the concentration of the HDL cholesterol. The method includes introducing a whole blood sample to a test device including a separation area comprising a first zone that separates the cellular components of the whole blood from the plasma or serum; and a second zone that separates the LDL and the VLDL fractions from the plasma or serum. The essentially cell-free, LDL-free and VLDL-free plasma or serum then migrates to a test area. After the plasma or serum migrates to the test area, plasma or serum is assayed for HDL cholesterol and the test area is examined for a quantitative response to HDL cholesterol present in the whole blood sample.

REFERENCES:
patent: 3092465 (1963-06-01), Adams et al.
patent: 3146163 (1964-08-01), Brewer
patent: 3298789 (1967-01-01), Hast
patent: 3552925 (1971-01-01), Fetter
patent: 3552928 (1971-01-01), Fetter
patent: 3607093 (1971-09-01), Stone
patent: 4045176 (1977-08-01), Proksch et al.
patent: 4059405 (1977-11-01), Sodickson et al.
patent: 4126416 (1978-11-01), Sears
patent: 4144306 (1979-03-01), Figueras
patent: 4188188 (1980-02-01), Willner et al.
patent: 4190628 (1980-02-01), Sears
patent: 4234317 (1980-11-01), Lucas et al.
patent: 4256693 (1981-03-01), Kondor et al.
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4366244 (1982-12-01), Pascal
patent: 4409106 (1983-10-01), Furuta et al.
patent: 4477575 (1984-10-01), Vogel et al.
patent: 4544630 (1985-10-01), Ziegenhorn et al.
patent: 4678757 (1987-07-01), Rapkin et al.
patent: 4746605 (1988-05-01), Kerscher et al.
patent: 4753776 (1988-06-01), Hillman et al.
patent: 4761381 (1988-08-01), Blatt et al.
patent: 4774192 (1988-09-01), Ferminiello et al.
patent: 4786603 (1988-11-01), Wiellinger et al.
patent: 4801687 (1989-01-01), Ngo
patent: 4810394 (1989-03-01), Masuda
patent: 4816224 (1989-03-01), Vogel et al.
patent: 4883765 (1989-11-01), Tamir et al.
patent: 4892815 (1990-01-01), Kerscher et al.
patent: 5064541 (1991-11-01), Jeng et al.
patent: 5110724 (1992-05-01), Hewett
patent: 5118613 (1992-06-01), McGowan
patent: 5135716 (1992-08-01), Thakore
patent: 5135719 (1992-08-01), Hillman et al.
patent: 5139685 (1992-08-01), de Castro et al.
patent: 5186843 (1993-02-01), Baumgardener et al.
patent: 5215886 (1993-06-01), Patel et al.
patent: 5262067 (1993-11-01), Wilk et al.
Clackson et al., "Precipitation of Serum Lipoproteins by Anionic Polyelectrolytes and Bivalent Cations", Monographs on Atherosclerosis, vol. II, 1982, pp. 17-39.

Affiliated with

Also associated with

Rating

Method of assaying whole blood for HDL cholesterol does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of assaying whole blood for HDL cholesterol, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of assaying whole blood for HDL cholesterol will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1886163