Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1984-08-09
1988-11-29
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 34, 435 39, 436531, 436534, C12Q 102, C12Q 104, C12Q 106, G01N 33546
Patent
active
047881429
ABSTRACT:
The activity of cells is assayed by mixing the cells capable of endocytosis with microbeads 0.03-20 .mu.m. in diameter containing a chemiluminecent substance and measuring the intensity of the luminescence generated by the action of highly reactive oxygen with the microbeads taken inside the cells.
REFERENCES:
Boros, Gradwohl's Clinical Laboratory Methods and Diagnosis, vol. 2, 8th Edition, The C. V. Mosby Co., St. Louis, Mo., 1249-1256 (1980).
Chemical Abstracts, vol. 92 No. 213341y, p. 453 (1980).
Mirro et al., J. Immunological Methods vol. 47: pp. 39-48 1981.
R. Allen, et al., "Phagocytic Activation of a Luminol-dependent Chemi-luminescence in Rabbit Aleolar and Peritoneal Macrophages," Biochem. Biophys. Res. Comm. 69: 245-252 (1976).
R. Allen, et al., "Evidence for the Generation of an Electronic Excitation State(s) in Human Polymorphonculear Leukocytes and its Participation in Bactericidal Activity," Biochem. Biophys. Res. Comm. 47: 679-684 (1972).
Hosaka Shuntaro
Uchida Takafumi
Kepplinger Esther M.
Toray Industries Incorporated
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