Method of assaying or analyzing subtypes of human leukocyte inte

Details Method of assaying or analyzing subtypes of human leukocyte inte Method of assaying or analyzing subtypes of human leukocyte inte

1990-12-04

1993-08-31

Rosen, Sam

Chemistry: analytical and immunological testing

Involving production or treatment of antibody

436543, G01N 33531

Patent

active

052408647

ABSTRACT:
Antiserum which can recognize all subtypes of human leukocyte interferon is prepared from the blood of an animal immunized with partially purified human leukocyte interferon obtained from a culture of human leukocyte stimulated with Sendai virus.
The antiserum is added to a column whereon concentrated culture broth of human leukocyte has been immobilized to adsorb impurities, the effluent is added to a column whereon partially purified human leukocyte interferon has been immobilized to adsorb anti-human leukocyte interferon antibody and then the antibody is eluted from the column.
The antibody thus obtained recognizes all subtypes of human leukocyte interferon and can be separated by a chromatography to each monoclonal antibody which recognizes a single subtype.
Employing these antibodies, the subtypes of human leukocyte interferon or their antibodies in a sample can be analized or assayed.

REFERENCES:
Dalton et al-Chem. Abst. vol. 96 (1982) p. 102 131t.

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