Method of assaying magnesium in human body fluid

Details Method of assaying magnesium in human body fluid Method of assaying magnesium in human body fluid

1991-04-24

1992-04-28

Lacey, David L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435810, 436 79, G01N 3350

Patent

active

051089050

ABSTRACT:
Disclosed is a method of assaying the content of Mg ions in a human body fluid such as serum, urine or saliva by the use of a reactant solution containing isocitrate dehydrogenase, NADP.sup.+, isocitrate and an excess amount of a chelating agent. Almost all Mg ions as existing in a human body fluid sample to be examined are bonded with the chelating agent, and the remaining Mg ions react with NADP.sup.+ to form NADPH. Increase of the thus formed NADPH is measured to obtain a standard curve of a straight line, and the content of Mg ions in the same is determined on the basis of the standard curve. If such an excess amount of a Mg-chelating agent is not added to the reactant solution, the intended standard could not be in the form of a straight line but is in the form of a tangent curve. Using the tangent curve, the content of Mg ions in the human body fluid sample cannot be assayed accurately.

REFERENCES:
patent: 4657854 (1987-04-01), Wegfahrt
patent: 4742001 (1988-05-01), Marui et al.

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