Method of assaying immunization stimulating activity

Details Method of assaying immunization stimulating activity Method of assaying immunization stimulating activity

1992-02-27

1994-03-01

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 724, 4351722, 435 701, 435 702, 435 7021, 435 703, 43524023, 435 704, 436 63, 436 64, 436547, 436548, 53038815, 5303888, G01N 33574, G01N 3353, G01N 33567

Patent

active

052906817

ABSTRACT:
A method of assaying immunization stimulating activity comprising (a) immunizing a lymphocyte in vitro by culturing the lymphocyte in contact with an immobilized antigen in a medium containing a test substance and (b) detecting an immunoglobulin, which is produced and secreted into the medium by the immunized lymphocyte and bound to the immobilized antigen, by counting the immunostained spots deposited in situ on the surface of the immobilized antigen.

REFERENCES:
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G. Kohler et al, "Continuous cultures of fused cells secreting antibody of predefined specificity", Nature, vol. 256, pp. 495-497, (1975) UK.
Ho et al, In Vitro Immunication of Human Lymphocytes, I. "Production of Human Monoclonal Antibodies Against Bombesin and Tetanus Toxoid", The J. of Immunol., vol. 135, No. 6, pp. 3831-3838 (1985) USA.
Teng et al, "Stragegies for Stable Human Monoclonal Antibody Production, Construction of Heteromyelomas, in Vitro Sensitization, and Molecular Cloning of Human Immunoglobulin Genes", ed. Engleman et al, Plenum Press, pp. 71-91 (1985) USA.
May-Kin Ho, "Production of Human Monoclonal Antibodies by In Vitro Immunization", ed. Strelkauskas (Marcel Dekker), pp. 23-38 (1987), USA.
Borrebaeck et al, "Human monoclonal antibodies produced by primary in vitro immunication of peripheral blood lymphocytes", Proc. Natl. Acad. Sci., vol. 85, pp. 3995-3999 (1988) USA.
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