Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1988-03-01
1991-05-28
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 79, 435 794, 435975, 436501, 436518, 436808, 436815, 436524, 436527, 436529, G01N 33535, G01N 3353, G01N 33566, G01N 33543
Patent
active
050194984
ABSTRACT:
A high molecular hyaluronic acid which is an important factor in the diagnosis of inflammations such as rheumatism or diseases such as cancer is assayed as a complex of sandwich structure in which a hyaluronic acid binding protein is coupled to the hyaluronic acid of interest at two or more sites of binding without the need to employ a competitive reaction as in the prior art techniques of assay. The assay method of the present invention does not require a purified form of hyaluronic acid as a reagent and permits as small as 10 ng of a high molecular hyaluronic acid to be detected or quantified by a very simple operation.
REFERENCES:
patent: 4826776 (1989-05-01), Brandt et al.
Alberts, B., et al., in Molecular Biology of the Cell, published by Garland Publishing, New York, NY (1983) pp. 702-703.
Oellerich, M., J. Clin. Chem. Clin. Biochem., vol. 22, 1984, pp. 895-904.
Analytical Bio Chemistry, vol. 109, 1980, pp. 386-394; Laurent et al, Determination of hyaluronate in biological samples by a specific radioassay technique.
Chemical Abstracts, vol. 107, No. 1, Jul. 1987, p. 345, col. 2, abstract No., Columbus, Ohio, U.S.; H. D. Keiser, A solid-phase immunoassay for the binding of cartiliage proteoglycan to hyaluronic acid, & ANAl. Biochem 1987, 160(2), 462-467.
Chemical Abstracts, vol. 88, No. 20, May 1978, p. 190, col. 1, abstract No. 147703g, Columbus, Ohio, U.S.; V. C. Hascall., Interaction of cartilage proteoglycans with hyaluronic acid, & J. Supramol. Struct. 1977, 7(1), 101-120.
Analytical Biochemistry. Determination of Hyaluronate in Biological Samples by a SpecificRadioassay Technique. 109, 386-394 (1980).
Analytical Biochemistry. Immunoenzymoassay of the Hyaluronic Acid-Hyaluronectin Interaction: Application to the Detection of . . . Cancer Patients. 149, 555-565 (1985).
Chugai Seiyaku Kabushiki Kaiska
Kepplinger Esther L.
Scheiner Toni R.
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