Method of assaying for agonists or antagonist of Dynorphin A...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007210, C530S350000

Reexamination Certificate

active

07078177

ABSTRACT:
The present invention is directed to assays that can be used to screen for compounds that act as agonists or antagonists or inverse agonists of Dynorphin A and its analogues. The assays are based upon the binding of Dynorphin A and its analogues to the rat and human MAS receptors.

REFERENCES:
patent: 5017689 (1991-05-01), Hruby et al.
patent: WO 9932519 (1999-07-01), None
Naqvi, et al. Structure-Activity Relationship Studies of Dynorphin A and Related Peptides. Peptides, 1998. 19(7): 1277-1292.
Ambroz et al. Dec. 3, 1991. The mas oncogene enhances angiotensin-induced [Ca2+]i responses in cells with pre-existing angiotensin II receptors. Biochim Biophys Acta. 1133(1): 107-111.
Tang et al. Dynorphin A elicits an increase in intracellular calcium in cultured neurons via a non-opioid, non-NMDA mechanism. J Neurophysiol. May 2000; 83(5): 2610-5.
Young et al. Jul. 1998. Characterization of the rat mas oncogene and its high-level expression in the hippocampus and cerebral cortex of rat brain. Proc Natl Acad Sci USA 85: 5339-5342.
Selbie et al, 1998. Trends in Pharmacol Sci. 19(3):87-93.
Kawanabe et al, 2003. Biochemical Pharmacology. 65: 1435-1439.
Michael Dumont et al., “Interactions of Dynorphin A and Related Peptides with Cardiac Ouabain Binding Sites,” J Mol Cell Cardiol, vol. 28, p. 615-621, (1996).
Thomas Walther et al., “Sustained Long Term Potentiation and Anxiety in Mice Lacking the Mas Protooncogene,” The Journal of Biological Chemistry, vol. 273 (No. 19), p. 11867-73, (1998).
Oliver Von Bohlen Und Halbach et al., “Interaction Between Mas and the Angiotensin AT1 Receptor in the Amygdala,” J. Neurophysiol., vol. 83 (No. 4), p. 2012-2021, (2000).
Mitsuyuki Ichinose et al., “Enhancement of phagocytosis by dynorphin A in mouse peritoneal macrophages,” Journal of Neuroimmunology, vol. 60, p. 37-43, (1995).

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