Method of assaying for acyl-L-carnitines and short-chain acyl-ca

Details Method of assaying for acyl-L-carnitines and short-chain acyl-ca Method of assaying for acyl-L-carnitines and short-chain acyl-ca

1991-10-09

1995-01-31

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

424 934, 435196, 4352521, 435829, 436815, A61K 3700, C12Q 144, C12N 916, C12N 120

Patent

active

053858298

ABSTRACT:
A method of assaying for acyl-L-carnitines including short chain acyl-carnitines including acetyl-L-carnitine and propionyl-L-carnitine in a substance, comprises subjecting a sample of the substance to be analyzed to an enzymatic hydrolysis using an acyl-carnitine esterase. The esterase is produced by Alcaligenes sp. FERM BP-2570 and it has substrate specificity for acyl-L-carnitines including short-chain acyl-L-carnitines. In addition the esterase demonstrates substrate specificity for acetyl-L-carnitine and propionyl-L-carnitine. The enzyme facilitates the hydrolysis reaction of one mole each of the acyl-L-carnitines with one mole of water in which to form one mole each of the corresponding fatty acid and L-carnitine. The amount of the fatty acid and L-carnitine formed is determined by this method.

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