Method of assaying a blood sample for prothrombin

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor

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435214, C12Q 156

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active

059358025

ABSTRACT:
An improved method of assaying undiluted whole blood or undiluted plasma for prothrombin is disclosed. The improved method utilizes carinactivase-1 and calcium ions, and eliminates the need for a prothrombin-deficient plasma as a reference plasma.

REFERENCES:
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patent: 5453370 (1995-09-01), Triplett et al.
patent: 5705198 (1998-01-01), Triplett et al.
Yamada et al., "Isolation and characterization of carinactivase, a novel prothrombin activator in Echis carinatus venom with a unique catalytic mechanism," The Journal of Biological Chemistry, vol. 271, No. 9, pp. 5200-5207, (1996).
Triplett et al., Procedures for the Coagulation Laboratory, Educational Products Divison American Society of Clinical Pathologists, Chicago, pp. 34-37 (1981).
Yamada D., Isolation and Characterization of Carinactivase, J of Biological Chemistry 271(9)5200-5207, 1996.
Yamada D., Prothrombin and Factor X Activator Activities in the Vernoms of Viperidae Snakes, Toxicon 35(11)1581-1589, 1997.
Francis J., A Rapid and Simple Micromethod for the Specific Determination of Descarboxylated Prothrombin, Medical Lab Sciences vol. 45 69-73, 1988.
Rob J., A Rapid and Highly Sensitive Chromogenic Microplate Assay for Quaitification of Rat and Human Prothrombin, Anal Biochem 245 222-225, Feb. 1997.

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