Method of assay of target nucleic acid

Details Method of assay of target nucleic acid Method of assay of target nucleic acid

2006-09-26

2006-09-26

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300

Reexamination Certificate

active

07112407

ABSTRACT:
A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once anda step of measuring a fluorescent signal in the presence of the reagent (I) at least once after addition of at least the reagents (A) to (H);(A) a first single-stranded oligonucleic acid complementary to a sequence neighboring the 5′ end of the specific nucleic acids sequence in the single-stranded RNA,(B) a second single-stranded oligo DNA complementary to a 3′-end sequence within the specific nucleic acids sequence,(C) an RNA-dependent DNA polymerase,(D) deoxyribonucleoside triphosphates,(E) a third single-stranded oligo DNA having (1) a promoter sequence for a DNA-dependent RNA polymerase, (2) an enhancer sequence for the promoter and (3) a 5′-end sequence within the specific nucleic acids sequence, in this order from the 5′ end,(F) a DNA-dependent DNA polymerase,(G) a DNA-dependent RNA polymerase,

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