Method of assay of enzymatic activity and apparatus therefor

Details Method of assay of enzymatic activity and apparatus therefor Method of assay of enzymatic activity and apparatus therefor

1995-06-06

1998-04-14

Chin, Christopher L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

356306, 356317, 356318, 356433, 356440, 356441, 422 55, 422 681, 422 8205, 422 8208, 4352871, 4352872, 4352887, 435808, 436172, 436805, G01N 33573

Patent

active

057389977

ABSTRACT:
Method of assay of enzymatic activity, comprising projecting excitation light to a sample containing an enzyme, a substrate which forms a product by action of the enzyme, and a reference substance which is insensitive to the action of the enzyme but emits fluorescence; obtaining a first measured value of fluorescence intensity of the sample at a first wavelength region which includes fluorescence emitted by the substrate or the product at least, obtaining a second measured value of fluorescence intensity at a second wavelength region which is different from the first wavelength region for the first measured value and includes fluorescence emitted by the reference substance; and assaying the enzymatic activity from the ratio of the first measured value to the second measured value and apparatus for performing the method. The method assures high accuracy and high sensitivity of measurement in enzyme labeled immunoassay and enzyme labeled DNA hybridization.

REFERENCES:
patent: 3741876 (1973-06-01), Guilbault et al.
patent: 4436827 (1984-03-01), Tamagawa
patent: 4617261 (1986-10-01), Sheldon, III et al.
patent: 4824778 (1989-04-01), Nagai et al.
patent: 4954435 (1990-09-01), Krauth
patent: 5143825 (1992-09-01), Chacko et al.

Affiliated with

Also associated with

Rating

Method of assay of enzymatic activity and apparatus therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of assay of enzymatic activity and apparatus therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of assay of enzymatic activity and apparatus therefor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-633771