Method of assay of enzymatic activity

Details Method of assay of enzymatic activity Method of assay of enzymatic activity

1992-06-18

1995-10-24

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 6, 435 79, 435183, 435967, 435968, 436164, 436172, 436536, 436537, 436805, G01N 33573

Patent

active

054609437

ABSTRACT:
Method of assay of enzymatic activity, including projecting excitation light to a sample containing an enzyme, a substrate which forms a product by action of the enzyme, and a reference substance which is insensitive to the action of the enzyme but emits fluorescence; obtaining a first measured value of fluorescence intensity of the sample at a first wavelength region which includes fluorescence emitted by the substrate or the product at least, obtaining a second measured value of fluorescence intensity at a second wavelength region which is different from the first wavelength region for the first measured value and includes fluorescence emitted by the reference substance; and assaying the enzymatic activity from the ratio of the first measured value to the second measured value and apparatus for performing the method. The method assures high accuracy and high sensitivity of measurement in enzyme labeled immunoassay and enzyme labeled DNA hybridization.

REFERENCES:
patent: 3741876 (1973-06-01), Guilbault, et al.
patent: 4617261 (1986-10-01), Sheldon III, et al.
patent: 4824778 (1989-04-01), Nagai et al.
patent: 4954435 (1990-09-01), Krayth
patent: 5143825 (1992-09-01), Chacko et al.

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