Method of assay for vitamin D metabolites

Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Separation of immune complex from unbound antigen or antibody

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436548, 436804, 436817, 436825, 935106, 935108, 935110, 435 68, G01N 33536, G01N 3382, G01N 3353, G01N 33531

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045857415

ABSTRACT:
The vitamin D metabolite, calcitriol, is determined in blood serum by radioimmunoassay employing monoclonal antibodies in which calcitroic acid serves as the immunogen.

REFERENCES:
patent: 4119647 (1978-10-01), Liebman et al.
patent: 4269777 (1981-05-01), De Luca et al.
Perry, III et al., Biochem. Biophys. Res. Comm. 112 (2), Apr. 29, 1983, 431-436.
Gray et al., Clin. Chem. 27(3) 458-463 (1981).
Dokoh et al., Anal. Biochem. 116, 211-222 (1981).
Eisman et al., Arch. Biochem. Biophys. 176, 235-243 (1976).
Gray et al., J. Clin. Endocrin. Metab. 299-306 (1977).
Perry et al., Internat. Workshop Vit. D., Williamsburg, Va., Feb. 1982, Abstracts, p. 70.

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