Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-07-23
1990-12-18
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
2041531, 204400, 204403, 204DIG5, 435 14, 435 25, 435817, 436526, 436806, 436536, 436501, C12Q 125, G01N 3353
Patent
active
049786105
ABSTRACT:
In its broadest aspect, the present invention provides a method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at leasts one electrode, in which method a labelled component of the assay medium is, at least in part, magnetically held in the vicinity of the electrode. The electrochemical assay method may include a separation step, whereby bound label may be separated from free label in the assay medium. Preferably the electrochemical assay method will include the step of determining a perturbation in an electrochemical characteristic of components of the assay medium associated with a ligand complexing reaction.
Such an assay method is applicable to, for example, antibodies and antigens and suitable labels include redox centers, enzyme labels in the presence of an electron-transfer mediator and electron-transfer mediator labels in the presence of an electron donor or acceptor.
REFERENCES:
patent: 4219335 (1980-08-01), Ebersole
patent: 4751053 (1988-06-01), Dodin et al.
"Magnetic Enzyme Membranes as Active Elements of Electrochemical Sensors Specific Amino Acid Enzyme Electrodes*", by C. Calvot et al., FEBS Letters, vol. 59, No. 2, Nov. 1975, pp. 258-262.
"Magnetic Enzyme Membranes as Active Elements of Electrochemical Sensors, Lactose, Saccharose, Maltrose Bienzyme Electrodes*", by M. Cordonnier et al., FEBS Letters, vol. 59, No. 2, Nov. 1975, pp. 263-267.
Forrest Gordon C.
Hill Hugh A. O.
Rattle Simon J.
Robinson Grenville A.
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