Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-01
1996-12-17
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435808, 435810, 435962, 436523, 436172, 436533, 436534, 436517, 436800, 436805, C12Q 168
Patent
active
055852416
ABSTRACT:
A method for the flow cytometric assay of an analyte using monodisperse particles carrying a specific binding partner, the analyte and binding partner being selected from the group consisting of (a) antigen and specific antibody, (b) hormone and hormone receptor, (c) hapten and antihapten, (d) polynucleotide and polynucleotide binding protein, (e) biotin and avidin or streptavidin, (f) enzyme and enzyme cofactor and (g) lectin and specific carbohydrate, and the method comprising the steps of adding to the aqueous sample a predetermined amount of particles and a predeterminded amount of a labelled ligand having affinity for the analyte or the binding partner and detecting and quantifying the resulting labelled ligand-carrying particles by a flow cytometer, the method using a pair of different particle types which are distinguishable from each other by the flow cytometer and which respectively carry binding partners having the same specificity but different binding affinity for the analyte and independently but simultaneously detecting the two types of labelled ligand-carrying particles by the flow cytometer.
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Saunders, G. Amplified Flow-Cytometric Separation--Free Fluorescence Immunoassays, 1985, 2020-2023.
Housel James C.
Nguyen Bao-Thuy L.
Sinvent A/S
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