Method of and system for analyzing a sample containing a...

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

Reexamination Certificate

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Details

C356S310000, C356S330000, C356S318000, C250S458100

Reexamination Certificate

active

06864975

ABSTRACT:
The present invention proposes a method of and system for analyzing samples (particularly, bio-samples), wherein the continuous spectrum white light pulse generated from an ultra-short optical pulse is used in a simple manner. By a system according to the invention, continuous spectrum white light pulses generated by converging ultra-short optical pulses on a point in a transparent substance23are dispersed into a spectrum of light by a dispersing element25,and a plurality of objective wavelength components are separately selected by small reflection mirrors37,masked mirrors or the like. The selected objective wavelength components are combined by the dispersing element25into a composite optical pulse containing the objective wavelength components. The composite optical pulse is irradiated onto the bio-sample28or other samples, whereby only the desired fluorescent substances are excited by the objective wavelength components corresponding to them. The selected objective wavelength components may preferably have different light path lengths. By this construction, the component optical pulses corresponding to the objective wavelengths in the composite optical pulse are separated from each other, so that the noise due to the interference of different wavelength components is prevented.

REFERENCES:
patent: 4807993 (1989-02-01), Nakamura et al.
patent: 6178041 (2001-01-01), Simon
patent: 6377344 (2002-04-01), Schoeppe
patent: 43 30 347 (1995-03-01), None
patent: 199 30 532 (2001-01-01), None
patent: 0 533 302 (1993-03-01), None
patent: 2352827 (2001-02-01), None
Weiner A.M.: “Femtosecond Pulse Shaping Using Spatial Light Modulators”, Review of Scientific Instruments, American Institute of Physics, New York, US, vol. 71, No. 5, May 2000 (pp. 1929-1960).

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