Method of and composite for fluorescent in situ hybridization

Details Method of and composite for fluorescent in situ hybridization Method of and composite for fluorescent in situ hybridization

1998-02-17

1999-02-16

Elliott, George C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536243, C12Q 168, C07H 2104

Patent

active

058719326

ABSTRACT:
A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorohores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (d) analyzing and presenting images of the now hybridized chromosome spread.

REFERENCES:
Lichter et al. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Human Genetics vol. 80, pp. 224-234, 1988.
Speicher et al. Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nature Genetics vol. 12 pp. 368-375, 1996.

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