Method of and apparatus for differentiating between normal and m

Details Method of and apparatus for differentiating between normal and m Method of and apparatus for differentiating between normal and m

1977-03-03

1978-12-26

Willis, Davis L.

Radiant energy

Luminophor irradiation

With ultraviolet source

G01N 2138

Patent

active

041318005

ABSTRACT:
The normality of a cell labelled with a lipid soluble fluorescent dye and excited with a beam of polarized light can be evaluated by measuring the time dependence of the state of polarization of fluorescence emitted by the cell which is determined by simultaneously measuring the intensities of fluorescence polarized in directions parallel and perpendicular to the direction of polarization of the excitation beam.
The state of polarization of a cell changes markedly as a function of excitation time when the cell is abnormal (i.e., malignant), while the state of polarization of a cell remains substantially constant when the cell is normal. Classification of cells in a population by way of the stability of the state of polarization provides guidance in determining the presence of malignancy in the population.

REFERENCES:
patent: 3887812 (1975-06-01), Hirschfeld
patent: 3971952 (1976-07-01), Inbar et al.

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