Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1990-11-30
1993-10-19
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 25, 435 30, 435 32, 435 39, 435287, 435291, 436150, 422 75, 422 76, 422 77, 422 81, 204 111, C12Q 104, G01N 2508
Patent
active
052544613
ABSTRACT:
A method of determining populations of live, whole bacteria electrochemically. The bacteria are filtered, and the filtrate is employed in association with an electrochemical measuring unit to determine the bacteria density. In accordance with a flow-through method, the average signal over the predetermined time period of the test is employed, in conjunction with a constant, to determine the population. In accordance with the bypass method, reagent is passed through a bypass line to the electrochemical measuring unit, and the resulting signal is subtracted from the signal resulting from the filtrate, a constant being employed to correlate the resulting remainder with bacteria count. A changing-concentration method employs a changing concentration of bacteria in the same fluid to determine populations of bacteria. A saved-sample method employs a second test of the same filtrate, after a predetermined time period, to compensate for any contaminants that may be present. Additional major embodiments relate to use of potential measurement to achieve the electrochemical measuring, to the measurement of rate of change of potential; and to a gas method of rapidly obtaining undiluted filtrate.
REFERENCES:
patent: 3228799 (1966-01-01), Rohrback
patent: 3403081 (1968-09-01), Rohrback et al.
patent: 3506544 (1970-04-01), Silverman et al.
patent: 3523070 (1970-08-01), Silverman et al.
patent: 3526578 (1970-09-01), Silverman
patent: 4200493 (1980-04-01), Wilkins et al.
patent: 4246343 (1981-01-01), Wilkins et al.
patent: 4252897 (1981-02-01), Axford et al.
patent: 4288543 (1981-09-01), Sieloff et al.
patent: 4321322 (1982-03-01), Ahnell
patent: 4386157 (1983-05-01), Nishioka et al.
patent: 4517291 (1985-05-01), Seago
patent: 4576916 (1986-03-01), Lowke et al.
patent: 4614716 (1986-09-01), Rohrback et al.
patent: 4777137 (1988-10-01), Lemonnier
patent: 4801546 (1989-01-01), Ackland
patent: 4983516 (1991-01-01), Turner et al.
patent: 5089395 (1992-02-01), Snyder et al.
R. G. Kroll, et al., Jul. 1, 1989, "An oxygen electrode-based assay of catalase activity as a rapid method for estimating the bacterial content of foods", Biological Abstracts, vol. 88, No. 1, (Abstract No. 3556, J. Appl. Bacteriol, 66(3), 209-218 (Eng).
Food Microbiology, vol. 6, No. 3, 1989, USA, pp. 159-169, R. A. Patchett et al. "Rapid detection of bacteria by an amperometric electrode system--a comparison of some redox mediators".
Journal of Applied Bacteriology, vol. 66, No. 1, 1989, Cambridge UK, pp. 49-55, R. A. Patchett et al. "Investigation of a simple amperometric electrode system to rapidly quantify and detect bacteria in foods".
Holmes Elmond A.
Rohrback Gilson H.
Gausewitz Richard L.
Infometrix, Incorporated
Mohamed Abdel A.
Wityshyn Michael G.
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