Method of and apparatus for determining hydrogen peroxide by ram

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 772, 435 4, 435 14, 435 25, 435 26, 435 27, 435 28, 435183, 435 71, 356301, G01N 3353

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057534497

ABSTRACT:
An excitation beam generated from a light source is separated into a measuring beam and a reference beam by an optical path adjusting optical system including a beam splitter, so that the measuring beam is applied to a sample in a cell. Raman scattering light which is generated from the sample is detected by a spectral detector including a spectroscope through a scattering light path adjusting optical system and a wavelength selector. In a spectrum obtained by the spectral detector, a peak of Raman scattering in which wavenumber shift from the wavelength of the excitation beam is present at 800 to 920 cm.sup.-1 is employed to make quantitative measurement of hydrogen peroxide. Hydrogen peroxide contained in an aqueous solution can be simply quantitatively analyzed through optical analysis means.

REFERENCES:
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patent: 4714345 (1987-12-01), Schrader
Chaudhuri et al Current Science (1989) 58(7) pp. 339-343, 1989.
"A Raman Study of H.sub.2 O.sub.2 and D.sub.2 O.sub.2 Vapor," Journal of Raman Spectroscopy, P.A. Giguere et al, vol. 2 (1974), pp. 125-132.
"Hydrogen Bonding in Hydrogen Peroxide and Water. A Raman Study of the Liquid State," Journal of Raman Spectroscopy, Paul A. Giguere et al., vol. 15, No. 3, 1984, pp. 199-204.

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