Method of and apparatus for classifying biological cells

Details Method of and apparatus for classifying biological cells Method of and apparatus for classifying biological cells

1977-03-29

1978-10-24

Willis, Davis L.

Radiant energy

Luminophor irradiation

With ultraviolet source

G01N 2138

Patent

active

041223489

ABSTRACT:
Cells in a population labelled with a lipid soluble fluorescent dye and excited with a beam of polarized light can be classified according to the distribution of the state of polarization of fluorescence emitted by a cell. The intensities of fluorescence, polarized in directions parallel and perpendicular to the direction of polarization of the excitation beam, are measured simultaneously to obtain the state of polarization.
The state of polarization, so determined, bears a functional relationship to the fluidity of the plasma membrane of the cell and is thus a measure of the cell's function. Consequently, classification of cells in a population by the distribution of the state of polarization of the fluorescence emitted in response to their excitation enables different types of cells to be distinguished according to biological function. For example, white blood cells can be differentially analyzed using the method of the present invention.

REFERENCES:
patent: 3497690 (1970-02-01), Wheeless, Jr. et al.
patent: 3896307 (1975-07-01), Trowe
patent: 3971952 (1976-07-01), Inbar et al.

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