Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-06-12
1998-08-25
Huff, Sheela
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 2, 435 724, 435962, 436526, 436236, 436546, 436548, 436 44, 436172, 436175, 356 39, G01N 33574, G01N 3353, G01N 33555, G01N 33567
Patent
active
057982171
ABSTRACT:
The invention provides a method for enriching a sample in tumor cells and determining the DNA ploidy and proliferative index of the tumor cells present in the sample. The method uses a pan-leukocyte monoclonal antibody conjugated to a separable substrate such as magnetic microspheres to conjugate and remove leukocytes from the sample. A fluorescently labelled monoclonal antibody specific to a tumor associated antigen or an antigen arising from the presence of the tumor and a DNA staining reagent are added to the leukocyte depleted sample. The sample is then analyzed by dual color flow cytometry to determine cells having an abnormal DNA content by gating on fluorescently labelled cells.
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Bolton Wade E.
Kenyon Norma Sue
Schmittling Robert J.
Siiman Olavi
Coulter Corporation
Huff Sheela
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