Method of analyzing intestinal flora and analytical apparatus

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C435S091520

Reexamination Certificate

active

06986991

ABSTRACT:
A method of analyzing intestinal bacteria flora which comprises: extracting a sample from a subject; extracting bacteria to prepare a bacteria suspension; extracting DNAs of bacteria from the bacteria suspension to prepare a DNA extract liquid; amplifying a specific region such as 16S rDNA using the DNA extract liquid; fractionating the amplified fragments by electrophoresis to obtain a fractional pattern; and comparing the fractional pattern with preliminarily obtained electrophoretic patterns of amplified fragments of intestinal bacteria flora, thereby analyzing the intestinal bacteria flora of the subject.

REFERENCES:
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5498392 (1996-03-01), Wilding et al.
patent: 5753467 (1998-05-01), Jensen et al.
patent: 5846783 (1998-12-01), Wu et al.
patent: 6274306 (2001-08-01), Inoue
patent: 6287769 (2001-09-01), Inoue
patent: 199 14 461 (1999-10-01), None
patent: 11-123093 (1999-05-01), None
patent: 11-127898 (1999-05-01), None
patent: 11-127899 (1999-05-01), None
patent: 11-151097 (1999-06-01), None
patent: WO 97/31256 (1997-08-01), None
Hamad et al. Journal of Applied Microbiology, 1997, vol. p. 746-770.
W. Day et al.; “Use of an Arbitrarily Primed PCR Product in the Development of a Campylobacter Jejuni-Specific PCR,” Applied and Environmental Microbiology; Mar. 1997; pp. 1019-1023.
C. Eichner et al.; “Thermal Gradient Gel Electorphoresis Analysis of Bioprotection From Pollutant Shocks in the Activated Sludge Microbial Community”, Applied and Environmental Microbiology; Jan. 1999; vol. 65, No. 1, pp. 102-109.
F. Schwieger et al.; “A New Approach to Utilize PCR-Single-Stranded-Conformation Polymorphism for 16S rRNA Gene-Based Microbial Community Analysis” Applied and Environmental Microbiology; Dec. 1998, vol. 64, No. 12, pp. 4870-4876.
M. Islam et al.; “Detection ofShigellaeFrom Stools of Dysentery Patients by Culture and Polymerase Chain Reaction Techniques”; J. Diarrhoel Dis. Res.; Dec. 1998, vol. 16, No. 4, pp. 248-251.
F. Ausubel et al.; “Current Protocols in Molecular Biology”; Supplement 9; 1990; pp. 2.4.1 to 2.4.2.
A. Hiraishi et al.; “Restriction Pattern Analysis by High-Performance Liquid Chromatography of PCR-Amplified 16S rDNA Fragments From Scum-Forming Bacteria in Activated Sludge”; Microbes and Environments; vol. 12; No. 3, 1997, pp. 57-68.
B. Alberts et al.; “Molecurlar Biology of the Cell”, Third Edition; p. 340.
“Lectures of Second Symposium of Japan Society of Water Environment” Sep. 13, 1999, pp. 54-55, and partial translation.
“Information Processing”; IPSJ Magazine; vol. 40, No. 3; Mar. 1999; pp. 320-325, and partial translation.

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