Method of analyzing components in biological samples

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol

Reexamination Certificate

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C435S019000, C435S025000, C435S026000, C435S028000, C435S975000

Reexamination Certificate

active

06986998

ABSTRACT:
There is provided a method for a selective assay of component, particularly cholesterol, in very low-density lipoprotein (VLDL) which is one of serum lipoproteins.In the assay, an enzymatic reaction of lipoprotein lipase (LPL) or cholesterol esterase (CE) which well reacts with high-density lipoprotein (HDL) and VLDL is carried out at least in the presence of calixarene or a salt thereof. It is also carried out in the presence of one or more substance(s) selected from albumin and basic amino acids in addition to calixarene or a salt thereof.

REFERENCES:
patent: 6114134 (2000-09-01), Kishi et al.
patent: 2003/0129681 (2003-07-01), Kishi et al.
patent: 1 020 532 (2000-07-01), None
patent: 9-43234 (1997-02-01), None
patent: 11-9300 (1999-01-01), None
patent: WO 98/59068 (1998-12-01), None
Hart et al, Infection & Immunity, V. 64(4), pp 1491-1493, (Apr., 1996).

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