Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Reexamination Certificate
2008-05-20
2008-05-20
Hobbs, Lisa J. (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
C435S133000, C424S009100, C514S002600, C514S690000
Reexamination Certificate
active
10537338
ABSTRACT:
The present invention relates to an analysis method and analysis system that can accurately analyze the contents of coenzyme Q-10 and a 2-electron reduced form thereof in a specimen, in which human blood plasma as the specimen is mixed with isopropyl alcohol as a pretreatment and the coenzyme Q-10 and the 2-electron reduced form thereof are extracted to the isopropyl alcohol. Extracted liquid is stored at a temperature of 4° C. until the analysis is performed. The extracted liquid as an analytical sample is analyzed by an analysis system provided with a liquid-sending mechanism, a switching mechanism, a concentration column, a separation column, a reduction column, an ultraviolet absorption detector, and an electrochemical detector.
REFERENCES:
patent: 2002/0125193 (2002-09-01), Tang et al.
patent: 0 956 854 (1999-11-01), None
patent: 55-039701 (1980-03-01), None
patent: 58-092609 (1983-06-01), None
patent: 61-293391 (1986-12-01), None
patent: WO 03/056024 (2003-07-01), None
Edlund, PO (1988) Determination of Coenzyme Q10, alpha tocopherol and cholesterol in biological samples by coupled-column liquid chromatography with coulometreic and ultraviolet detection. J Chromatogr B 425: pp. 87-97.
Grossi G et al (1992) Improved high-performance liquid chromatographic method for the determination of coenzyme Q10 in plasma. J Chromatogr 593: pp. 217-226.
Mosca F et al (published online Apr. 25, 2002) Assay of coenzyme Q10 in plasma by a single dilution step. Anal Biochem, vol. 305, pp. 49-54.
Yamashita, S., et al., “Simultaneous Detection of Ubiquinol and Ubiquinone in Human Plasma as a Marker of Oxidative Stress,”Analytical Biochemistry, 250 (1997), pp. 66-73.
Edlund, P., “Determination of Coenzyme Q10α-Tocopherol and Cholesterol in Biological Samples by Coupled-Column Liquid Chromatography with Coulometric and Ultraviolet Detection,”Journal of Chromatography, 425(1988) pp. 87-97.
Mita Masashi
Yamamoto Yorihiro
Hobbs Lisa J.
Shiseido Company Ltd.
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