Method of analysis of samples by determination of a function...

Radiant energy – Luminophor irradiation – Methods

Reexamination Certificate

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Reexamination Certificate

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07019310

ABSTRACT:
A method for characterizing samples having units, by monitoring fluctuating intensities of radiation emitted, scattered and/or reflected by said units in at least one measurement volume, the monitoring being performed by at least one detection means, said method comprising the steps of:a) measuring in a repetitive mode a number of photon counts per time interval of defined length,b) determining a function of the number of photon counts per said time interval,c) determining a function of specific brightness of said units on basis of said function of the number of photon counts.

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H. Quian, et al.,Proc. Natl. Acad. Sci., vol. 87, Jul. 1990, pp. 5479-5483.
SM. NIE, et al.,Analytical Chemistry, vol. 67, No. 17, Sep. 1, 1995, pp. 2849-2857.
Rigler et al. “Fluorescence correlation spectroscopy with high count and low background: analysis of translational diffusion.” Eur. Biophys. J. (1993) 22: 169-175.

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