Image analysis – Applications – Biomedical applications
Reexamination Certificate
2011-02-01
2011-02-01
Tucker, Wesley (Department: 2624)
Image analysis
Applications
Biomedical applications
C382S134000, C435S004000
Reexamination Certificate
active
07881517
ABSTRACT:
A method is provided for comparing multiple samples of cell extract containing a plurality of components. The method comprises the steps of preparing at least two samples of cell extract from at least two groups of cells and of exposing each of said sample of said cell extract to a different one of a set of matched markers, e.g. luminescent markers, to bind the marker to the cell extract to label the cell extract, each marker within said set of markers being capable of binding to the cell extract and can be individually detected from all other markers within said set. The samples are then mixed to form a mixture and said mixture is electrophoresed to separate the components within the cell extract. At least two electronic images of the electrophoresed mixture are obtained (I) by detection of the individual markers, each image being represented by detection of a marker different from the others. One resultant electronic image (Ires) of the obtained at least two electronic images is created (II) and analyzed in order to identify spot analysis areas (III). The identified spot analysis areas are applied on the respective at least two electronic images for evaluating said areas in order to detect spots representing components of said cell extracts (IV).
REFERENCES:
patent: 5235522 (1993-08-01), Bacus
patent: 5694478 (1997-12-01), Braier et al.
patent: 6043025 (2000-03-01), Minden et al.
patent: 6535624 (2003-03-01), Taylor, Jr.
patent: 6611766 (2003-08-01), Larsen et al.
patent: 7155050 (2006-12-01), Sloge et al.
patent: WO96/33406 (1996-10-01), None
Bettens, E., et al., “Computer Analysis of Two-Dimensional Electrophoresis Gels: A New Segmentation and Modeling Algorithm”, Electrophoresis, 1997, vol. 18, p. 792-798.
Thompson, A., et al., “Information Extraction from 2D Electrophoresis Images”, Proceedings of the 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, vol. 20, No. 2, 1998, p. 1060-1063.
Watanabe, K., et al., “Automated Detection and Matching of Spots in Autoradiogram Images of Two-Dimensional Electrophoresis for High-Speed Genome Sequencing”, Kanazawa Institute of Technology, IEEE, 1997, p. 496-499.
Björkesten Lennart
Sloge Erik
Soderman Tobias
GE Healthcare Bio-Sciences AB
Ji Yonggang
Tucker Wesley
LandOfFree
Method of analysing cell samples, by creating and analysing... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of analysing cell samples, by creating and analysing..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of analysing cell samples, by creating and analysing... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2641858