Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1996-07-01
1998-09-08
Railey, II, Johnny F.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435106, 435115, 4351723, 43525232, C12N 121, C12P 1304, C12P 2100, C12P 2104
Patent
active
058044140
ABSTRACT:
A method of amplifying a desired gene in a chromosome of a coryneform bacterium, which comprises forming an artificial transposon in which a drug resistance gene and the desired gene are inserted into an insertion sequence of the coryneform bacterium, and introducing said artificial transposon into the coryneform bacterium. In accordance with the method of the present invention, a desired gene can be amplified in a chromosome in coryneform bacteria which are used in the industrial production of amino acids or nucleic acids.
REFERENCES:
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Tauch et al., Plasmid 33:168-179 (1995).
Tauch et al., Plasmid 34:119-131 (1995).
Molecular Microbiology, vol. 11, No. 4, pp. 739-746, 1994, Alain A. Vertes, et al., "Isolation and Characterization of IS31831, a Transposable Element from Corynebacterium Glutamicum".
Molecular Microbiology, vol. 14, No. 3, pp. 571-581, 1994, Celine Bonamy, et al., "Identification of IS1206, a Corynebacterium Glutamicum IS3-related Insertion Sequence and Phylogenetic Analysis".
Mol Gen Genet, vol. 245, pp. 397-405, 1994, Alain A. Vertes, et al., "Transposon Mutagenesis of Coryneform Bacteria".
FEMS Microbiology Letters, vol. 126, pp. 1-6, 1995, Wolfgang Jaeger, et al., "Isolation of Insertion Elements from Gram-Positive Brevibacterium, Corynebacterium and Rhodococcus Strains Using the Bacillus Subtilis sacB Gene as a Positive Selection Marker".
Hayakawa Atsushi
Hirano Seiko
Izui Masako
Matsui Hiroshi
Moriya Mika
Ajinomoto Co. Inc.
Railey II Johnny F.
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