Method of amplification using intermediate renaturation step

Details Method of amplification using intermediate renaturation step Method of amplification using intermediate renaturation step

1994-06-22

1996-09-24

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 5, 536 243, 536 2433, C12P 1934, C12Q 168, C12Q 170, C07H 2104

Patent

active

055590136

ABSTRACT:
A method for amplification and detection of a low copy target nucleic acid includes coamplification of a high copy target nucleic acid. After a number of conventional amplification cycles which include a denaturation step, several cycles are carried out during which the denatured products are renatured for a brief period of time. This intermediate step in later cycles of the amplification process reduces the effective concentration of the high copy target nucleic acid available for amplification in later cycles, thereby making more DNA polymerase available for amplification of the low copy target nucleic acid.

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Gautelli et al., Clin. Microbiol. Rev., 2 (2), pp. 217-226 (1989).
Pang et al., Nature, 343 (4), pp. 85-89 (1990).

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