Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-08-30
2005-08-30
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
06936420
ABSTRACT:
A method of amplification of one or more DNA sequences in a DNA containing sample, and methods of investigating such samples are provided in which an improved amplification cycling regime is used. The regime features a first phase and a second phase, the first phase including a denaturation temperature step, followed by an annealing temperature step, followed by a corrected annealed primer extension temperature step, these steps being followed by an annealing temperature step, corrected annealed primer extension temperature step prior to any further denaturation temperature steps being applied. Increased amplification from a given number of amplification cycles and maximised sensitivity for the DNA amplification process is achieved as a result.
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Wu et al., “The Effect of Temperature and Oligonucleotide Primer Length on the Specificity and Efficiency of Amplification by the Polymerase Chain Reaction”, DNA and Cell Biology, vol. 10, No. 3, pp. 233-238, Apr. 1991.
Sugimoto et al., “Quanatative Detection of DNA by Coamplification Polymerase Chain Reaction: A wide Detectable Range Controlled by the Thermodynamic Stability of Primer Template Duplexes”, Analytical Biochemistry, vol. 211, No. 1, pp. 170-172, May 1993.
Backus et al., “Method of Amplification Using Intermediate Renaturation Step”, Biotechnology Advances Research Reviews and Patent Abstracts vol. 15 No. 2, 1997 Abstract.
Gill Peter David
Hussain Javaid Iqbal
Long Adam Spencer
Tully Gillian
Horlick Kenneth R.
Merchant & Gould P.C.
The Secretary of State for the Home Department
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