Method of amino acid analysis

Details Method of amino acid analysis Method of amino acid analysis

1984-09-11

1987-06-02

Richman, Barry S.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436 89, 436164, 436172, G01N 2177, G01N 3352, G01N 3368

Patent

active

046704035

ABSTRACT:
A method of amino acid analysis by reacting an amino acid-containing sample with o-phthalaldehyde and an N-protective group-substituted cysteine such as N-acetyl-L-cysteine under an alkaline condition to yield a fluorescent compound and measuring the intensity of fluorescence or absorbance of the resulting compound. The method is sensitive for quantitative and qualitative analysis of a secondary amino acid such as proline when it is beforehand subjected to an oxidation.

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