Method of altering enzymes and a novel neopullulanase

Details Method of altering enzymes and a novel neopullulanase Method of altering enzymes and a novel neopullulanase

1994-11-14

1999-10-12

Wax, Robert A.

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

435 691, 435193, 435201, 435210, 4352523, 4353201, 435476, 536 232, C12N 1511, C12N 1554, C12N 1556, C12N 1570

Patent

active

059654422

ABSTRACT:
An enzyme is altered by introducing an additional hydrophobic group to enhance the hydrophobic environment in the enzyme so as to interfere with entrance of a water molecule. In this connection, the site of introduction and kind of hydrophobic group to be introduced are selected on the basis of comprehensive analysis of amino acid sequence, three-dimensional structure, reaction mechanism or the like of the target enzyme. Using this method, an amino acid residue of neopullulanase derived from Bacillus stearothermophilus was replaced by another amino acid.

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