Method of alleviating chronic pain via peripheral...

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing

Reexamination Certificate

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C514S439000, C514S456000, C514S558000, C514S561000

Reexamination Certificate

active

10245098

ABSTRACT:
A composition having sustained pain-relieving properties such that the composition may be administered to a subject to alleviate chronic pain. The composition includes an effective amount of at least one glutaminase inhibitor. A method for alleviating chronic pain in a subject for an extended period of time is also disclosed, in which the compound is administered to a subject suffering from chronic pain at a site of inflammation such that the administration of the compound results in a reduction in at least one of thermal and mechanical pain responses at the site of inflammation for a period of at least two days without any resulting acute pain behavior. The composition may further include an effective amount of at least one compound having analgesic effects such that the composition also alleviates acute pain.

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Campos, J. A. et al, Involvement of Essential Cysteine and Histidine Residues in the Activity of Isolated Glutaminase from Tumour Cells. Biochim. Biophys. Acta. 1998, vol. 1429, pp. 275-283.
Kenneth E. Miller et al., “Glutamine-, glutamine synthetase-, glutamate dehydrogenase- and pyruvate caboxylase-immunoreactivities in the rate dorsal root ganglion and peripheral nerve”, Brain Research, vol. 945, pp. 202-211, (2002).
Taira et al.; “Effect of intrathecally administered protease inhibitors on the capsaicin-induced nociceptive behavior in mice”; XP002365320 retrived from STN; Database accession No. 127:344461; *abstract* & Annual Report of the Tohoku College of Pharmacy, 43, 139-145, CODEN: TYKNAQ; ISSN: 0495-7342 (1996).

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