Method of aligning, compensating, and calibrating a flow cytomet

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435967, 436 8, 436 10, 436800, 436 63, 436808, 356318, 356441, 356213, 356243, G01N 3353, G01N 3100, G01N 3348, G01J 330

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active

050932349

ABSTRACT:
A kit of highly uniform size microbead standards for flow cytometer alignment, compensation, and/or calibration, comprising a blank microbead population and/or an auto-fluorescent microbead population, together with two or more series of calibrated microbead populations labeled with fluorescent dye(s) which (i) prior to fluorescent dye(s) labeling, match the fluorescence spectra and fluorescence intensity of the blank and/or autofluorescent microbead population, and (ii) after fluorescent dye(s) labeling, match the fluorescence spectra and fluorescence intensity of fluorescently labeled samples to be measured on the flow cytometer. Also disclosed is a corresponding method to align, compensate, and/or calibrate a flow cytometer so as to make measurements on samples comparable and independent of the specific instrument and instrument settings.

REFERENCES:
patent: 4162282 (1979-07-01), Fulwyler et al.
patent: 4767206 (1988-08-01), Schwartz
patent: 4774189 (1988-09-01), Schwartz
patent: 4857451 (1989-08-01), Schwartz

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