Method of adsorbing viruses from fluid compositions

Details Method of adsorbing viruses from fluid compositions Method of adsorbing viruses from fluid compositions

1995-09-22

1997-08-19

Zitomer, Fred

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

Recovery or purification

4352351, 5253274, 5253276, 525380, 525382, 525420, 530421, 530418, C12N 702

Patent

active

056587791

ABSTRACT:
A method of adsorbing from a solution comprising a biological sample viruses which retain their viability and infectivity. The method comprises adjusting the pH of said solution to pH 6.0 to 8.0; adding an effective amount of a water insoluble cross-linked polycarboxylic acid polymer ("WCPP") into said solution in a volume:volume ratio of WCPP to solution of 100:1 to 1:10,000 to form a WCPP-solution mixture; incubating said WCPP-solution mixture for a time sufficient to immobilize said viruses on said WCPP forming a WCPP-virus matrix; and separating said matrix from said solution. This novel method is suitable for removing, purifying, recovering and analyzing viable viruses as well as viral components such as viral proteins and nucleic acids.

REFERENCES:
patent: 3684777 (1972-08-01), Field
patent: 4271028 (1981-06-01), Marfurt
patent: 4421653 (1983-12-01), Le Dain

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