Method of administering proteins to living skin cells

Details Method of administering proteins to living skin cells Method of administering proteins to living skin cells

1991-01-23

1993-03-02

Patterson, Jr., Charles L.

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Liposomes

424 945, 435193, A61K 3722, A61K 3752

Patent

active

051907623

ABSTRACT:
A method for administering a protein having intracellular biological activity into the interior of living skin cells, which lie below the skin's stratum corneum, is provided. The method comprises the steps of: (a) encapsulating the protein in liposomes; and (b) applying the liposomes to the outer surface of living skin so that the protein encapsulated in the liposomes traverses the skin's stratum corneum and the outer membranes of said cells and is thereby delivered by the liposomes into the interior of said cells. In certain preferred embodiments, the liposomes are pH sensitive liposomes. In other preferred embodiments, the protein is a DNA repair enzyme, such as T4 endonuclease V.

REFERENCES:
patent: 4356167 (1982-10-01), Kelly
patent: 4789633 (1988-12-01), Huang et al.
Ceccoli et al., The Journal of Investigative Dermatology, vol. 93, pp. 190-194, 1989.
Yarosh, Daniel B., abstract of meeting of Society of Cosmetic Chemists, New York, Dec. 7-8, 1989.
Scopes, Robert K., Protein Purification: Principles and Practice, Springer-Verlag, New York, pp. 152-155, 1982.
Liposome Technology, G. Gregoriadis ed., CRC Press, Boca Raton, Florida, 1984, vol. II, Chap. 12, pp. 178-185, and Chap. 15, pp. 207-221.
Repair of DNA Lesions Introduced by N-Nitroso Compounds, B. Myrnes & H. Krokan eds., Norwegian Univ. Press, Oslo, 1986, pp. 112-134.
Yasuda et al., Biochimicia et Biophysica Acta, 1976, vol. 442, pp. 197-207.
Cell Fusion, edited by A. E. Sowers, Plenum Press, N.Y., 1987, Chapter 11, pp. 241-267.
DNA Repair, edited by Friedberg et al., vol. I, Part A, chapters 18, 20-22; vol. II, chapters 3-5; vol. III, Section IV, chapters 23-25.
Yarosh et al., Molecular and Cellular Biology, 1981, vol. 1, pp. 237-244.
Yarosh, Mutation Research, 1985, vol. 145, pp. 1-16.
Yasuda et al., Proceedings of the National Academy of Sciences, Dec., 1970, vol. 67, pp. 1839-1845.
Westerhof, W., Medical Hypotheses, vol. 16, pp. 283-288, 1985.
Wohlrab W. and Lasch, J. Dermatologica, vol. 174, pp. 18-22, 1987.
Yamaizumi et al., Mutation Research, vol. 217, pp. 135-140, 1989.
Valerie et al., Cancer Research, 1987, vol. 47, pp. 2967-2971.
Vermorken, et al., J. Pharm. Pharmacol., vol. 36, pp. 334-336, 1984.
Warner et al., Journal of Virology, 1981, vol. 40, pp. 204-210.
Strauss, G., J. Soc. Cosmet. Chem, vol. 40, pp. 51-60, 1989.
Tanaka et al., Proceedings of the National Academy of Sciences U.S.A., 1975, vol. 72, pp. 4071-4075.
Tanaka et al., Proceedings of the National Academy of Sciences U.S.A., 1977, vol. 74, pp. 2958-2962.
Shek, et al., Biochimica et Biophysica Acta, vol. 902, pp. 229-236, 1987.
Straubinger, R., Hong, K., Friend D., and Papahadjopoulos, D., Cell, vol. 32, pp. 1069-1079, 1983.
Straubinger, R. Duzgunes N. and Papahadjopoulos, D. FEBS Letters, vol. 179, pp. 148-154, 1985.
Patel, H. M. Biochemical Society Transactions, vol. 13, pp. 513-516, 1985.
Robbins et al., Annals of Internal Medicine, vol. 80, No. 2, pp. 221-248, Feb., 1974.
Seawell et al., in DNA Repair, edited by Friedberg et al., N.Y., 1981, vol. 1, Part A, pp. 229-236.
Nakabeppu et al., Proceedings of the National Academy of Sciences, 1981, vol. 78, pp. 2742-2746.
Nakabeppu et al., Journal of Biological Chemistry, 1982, vol. 257, pp. 2556-2562.
Ostro, "Liposomes", Scientific American, Jan. 1987, vol. 256, pp. 102-111.
Myrnes et al., Eur. J. Biochem., vol. 173, No. 2, pp. 383-387, 1988.
Myrnes et al., Carcinogenesis, 1984, vol. 5, pp. 1061-1064.
Nakabeppu et al., The Journal of Biological Chemistry, vol. 260, pp. 7281-7288, 1985.
Mezei, M. and Gulasekharam, V. Life Sciences, vol. 26, pp. 1473-1477, 1980.
Mezei, M. and Gulasekharam, V. Journal of Pharmacy and Pharmacology, vol. 34, pp. 473-474, 1981.
Mosmann, Journal of Immunological Methods, vol. 65, pp. 55-63, 1983.
Jacobs, et al., J. Pharm., Pharmacol., vol. 40, pp. 829-833, 1988.
Komatsu, et al., Chem. Pharm. Bull., vol. 34, pp. 3415-3422, 1986.
McMillan et al., Journal of Virology, 1981, vol. 40, pp. 211-223.
Higgins et al., Mutation Research, 1987, vol. 183, pp. 117-121.
Ho, et al., Journal of Controlled Release, vol. 2, pp. 61-65, 1985.
Hoeijmakers, Journal of Cell Science Suppl., 1987, vol. 6, pp. 111-125.
Friedberg et al., Journal of Bacteriology, 1971, vol. 106, pp. 500-507.
Ganesan, et al., International Journal of Pharmaceutics, vol. 20, pp. 139-154, 1984.
Gesztes, A. and Mezei, M. Anesthesia and Analgesia, vol. 67, pp. 1079-1081, 1988.
Demple et al., The Journal of Biological Chemistry, vol. 257, pp. 13776-13780, 1982.
Ellens, Bentz and Szoka, Biochemistry, vol. 23, pp. 1532-1538, 1984.
Freeman et al., Analytical Biochemistry, vol. 158, pp. 119-129, 1986.
Bentz, Ellens and Szoka, Biochemistry, vol. 26, pp. 2105-2116, 1987.
Chenevert et al., Molecular and General Genetics, 1986, vol. 203, pp. 163-171.
Ciarrocchi et al., Proceedings of the National Academy of Sciences U.S.A., 1978, vol. 75, pp. 1887-1891.

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