Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Patent
1994-02-04
1999-07-27
Campell, Bruce R.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
4351721, 4351723, 424 931, 424 932, 424450, 514 44, 935 55, 935 56, 935 57, A61K 4800, A61K 9127, A01N 6300, C12N 500
Patent
active
059289449
ABSTRACT:
The present invention provides an adenoviral-mediated method of transfection with nucleic acids which can be augmented through incubation of the nucleic acids with cationic agents. Specifically, the present inventive method of introducing a nucleic acid into a eukaryotic cell comprises contacting the cell with, in any order or simultaneously, the nucleic acid and an adenovirus, wherein the nucleic acid is not bound to any molecule capable of effecting its entry into the cell. The cell is preferably additionally contacted with a cationic agent, such as a monocationic or polycationic liposome, such that the nucleic acid is not bound to any molecule capable of effecting its entry into the cell other than, optionally, the cationic agent.
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Crystal Ronald G.
Rosenfeld Melissa
Seth Prem
Yoshimura Kunihiko
Campell Bruce R.
The United States of America as represented by the Department of
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