Method of adapting anchorage-dependent cell lines to suspension

Details Method of adapting anchorage-dependent cell lines to suspension Method of adapting anchorage-dependent cell lines to suspension

1993-03-12

1994-09-20

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524025, 43524023, 4352402, C12N 500, C12N 502

Patent

active

053488776

ABSTRACT:
Normally anchorage-dependent insect cell lines are adapted to replicate under suspension conditions by addition of heparin to the culture medium and selection for resulting suspension-tolerant cells.

REFERENCES:
patent: 5024947 (1991-06-01), Inlow et al.
Karlsson et al, Laboratory Investigation, 60(5): 659-666, (1989).
Oberlander et al, Archives of Insect Biochemistry and Physiology, 5(2): 81-89, (1987), Abstract.
Carroll, Ippolito, and DeWolf "Heparin-Binding Agglutinin on Human Teratocarcinoma Cells", Biochem. Biophys. Res. Commun., 1982, 109/4 (1353-1359).
Thurn and Underhill, Heparin-Induced Aggregation of Lymphoid Cells; J. Cell Physiol., 1986, 126/3 (352-358).
Gill, Silbert and Silbert, Effects of Heparin Sulfate Removal on Attachment and Reattachment of Fibrobalsts and Endothelial Cells; Biochemistry, 1986, 25/2 (405-410).
Moore, Mahaffey, and Zboran, Heparin-Induced Agglutination of Erythrocytes in Horses; American Journal of Veterinary Research 48 (1). 1987: 68-71.
Mahaffey and Moore, Erythrocyte Agglutination Associated with Heparin Treatment in Three Horses J. American Veterinary Medical Association 1986. 189 (11): 1478-1480.
Kozlov, Kaznacheev, Lozovoi, and Lyakhovich, Mechanism of Action of Heparin of Lymphocytes in Vitro; Bull. Exp. Biol. Med., 1978, 84/11 (1612-1614).
Dougherty, et.al.--"Physical Factors That Affect In Vitro Autographica californica Nuclear Polyhedrosis Virus Infection" (Applied and Environmental Microbiology, May 1981, pp. 1166-1172).

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