Method of activating hydroxyl groups of a polymeric carrier usin

Details Method of activating hydroxyl groups of a polymeric carrier usin Method of activating hydroxyl groups of a polymeric carrier usin

1984-12-07

1986-04-15

Kight, John

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

525 541, 435178, 435179, 435180, 435181, 260112R, 260112G, C08F28300, C12N 1106

Patent

active

045828750

ABSTRACT:
Organic ligands containing at least one primary or secondary amino group or sulfhydryl group are coupled to a polymeric carrier containing at least one hydroxyl group, by activating the polymeric carrier by treatment with 2-fluoro-1-methylpyridinium toluene-4-sulfonate and reacting the activated polymeric carrier with the organic ligand which is thereby bonded directly to a carbon atom of the polymeric carrier. The polymeric carrier can, for example, be a possibly cross-linked polysaccharide and the organic ligand is preferably a biologically active material such as a drug or protein. The coupling product is an affinity matrix which can be used for affinity purification, covalent chromatography and reversible or irreversible covalent immobilization of biologically active molecules. The coupling method can be performed under mild conditions not damaging sensitive molecules.

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