Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1995-09-12
1997-07-22
Tran, Lien
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 29, 435 38, 435 39, 4352521, 4352528, 4352536, 435848, 435849, C12Q 104
Patent
active
056502907
ABSTRACT:
A selective culture medium which permits simultaneous detection of total coliform and Escherichia coli in a test sample with a single growth phase incubation period. The culture medium includes the required components of: (i) carbon nutrients, (ii) a nitrogen nutrient, (iii) a source of metabolizable potassium, (iv) a source of metabolizable phosphate, (v) vitamins, (vi) minerals, (vii) amino acids, (viii) sodium pyruvate, (ix) a bactericidal system selective for non-coliform bacteria, and (x) a sensible indicator selectively metabolized by Escherichia coli to the exclusion of other coliforms; and the optional components of: (xi) a source of metabolizable magnesium, (xii) sodium chloride as a recovery agent, (xii) a nonionic surfactant, (xiii) a contrast promoter effective for enhancing the color differentiation between Escherichia coli colonies growing in the medium and other bacterial colonies growing in the medium induced by the sensible indicator, and (xiv) a highlighting dye for enhancing the visual differentiation of coliform colonies relative to the culture medium.
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Hach Company
Tran Lien
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