Method in assaying methods involving biospecific affinity reacti

Details Method in assaying methods involving biospecific affinity reacti Method in assaying methods involving biospecific affinity reacti

1978-03-02

1980-11-04

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 435 7, 424 12, G01N 3316, A61K 4300

Patent

active

042319994

ABSTRACT:
A method of carrying out assaying methods involving biospecific affinity reactions in which there is used a component (I), which is labelled with at least one analytically indicatable group and is soluble in the liquid in whose presence the biospecific affinity reaction is carried out, for reaction with a counterpart (II) which exhibits biospecific affinity to (I) and optionally also one or more additional counterparts exhibiting biospecific affinity to (I) and/or (II), and forming an insoluble conjugate in which the labelled component (I) is incorporated and which conjugate is separated from the liquid phase, whereafter the analytically indicatable group is assayed in the insoluble conjugate, said method being characterized in that the analytically indicatable group is bound to the component (I) with a splittable bond of covalent nature and/or one of said counterparts is bound to a carrier, which is insoluble in said liquid, with a splittable bond of covalent nature, said splittable bonds being splittable under conditions which have practically disturbing influence on the assay method used; and in that the insoluble conjugate is split at said splittable bond or bonds, in the presence of a liquid, to form fragments which are soluble in said liquid and which contain the analytically indicatable group, which group is then assayed in the liquid phase.

REFERENCES:
patent: 3896217 (1975-07-01), Johnson
patent: 4041146 (1977-08-01), Giaever
patent: 4108975 (1978-08-01), Hales
patent: 4108976 (1978-08-01), Reese

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