Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1989-08-18
1991-03-12
Teskin, Robin
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
435 721, 4352401, 4352402, 436501, 436503, 436510, 436514, 436547, 436548, 436811, 436813, 436824, 436906, 530413, A01N 102, C12Q 100, C12N 500
Patent
active
049992830
ABSTRACT:
A method for separating male and female determining spermatozoa includes the initial step of exposing freshly ejaculated spermatozoa in a substantially protein free diluent to an excess concentration of a monoclonal antibody directed against H-Y antigen that binds substantially exclusively with male determining spermatozoa. The method continues with the suspending of the exposed spermatozoa together with a conjugate of (1) an immunoglobulin G antibody that binds substantially exclusively to the monoclonal antibody and (2) an immunoabsorbant substrate in a substantially protein free diluent. This forms a conjugate/spermatozoa preparation. The method concludes with the recovering of the separated male and female determining spermatozoa.
REFERENCES:
patent: 3894529 (1975-07-01), Shrimpton
patent: 4083957 (1978-04-01), Lang
patent: 4276139 (1981-07-01), Lawson
patent: 4448767 (1984-05-01), Bryant
patent: 4511661 (1985-04-01), Goldberg
patent: 4680258 (1987-07-01), Hammerling et al.
"H-Y Antigen: Genetics and Serology", 1977 Immunological Review, Wachtel.
Bennett & Boyse, "Sex Ratio in Progeny of Mice Inseminated with Sperm Treated with H-Y Antiserum".
Dawson Karl A.
Zavos Panayiotis M.
Teskin Robin
University of Kentucky Research Foundation
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