Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy...
Reexamination Certificate
1995-01-23
2001-06-12
Beisner, William H. (Department: 1744)
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
C435S264000, C210S606000
Reexamination Certificate
active
06245552
ABSTRACT:
BACKGROUND OF THE INVENTION
(1) Field of the Invention
The present invention relates to a method for providing microorganisms in an organic waste system for degrading organic wastes into carbon dioxide and water. In particular, the present invention relates to a method wherein the microorganisms are grown on site in a container for up to about 32 hours prior to addition to the waste system. The microorganisms after being grown on site on the growth medium to a level of at least about 10
9
cells/ml can be added to the organic wastes to degrade them at a sufficient dosage, usually at about 10
6
cells/ml of organic waste.
(2) Prior Art
Organic compounds have been shown to be degraded as described in the following references: U.S. Pat. No. 4,452,894 to Olsen et al; U.S. Pat. No. 4,593,003 to Vandenbergh; Vandenbergh, P. A. and A. M. Wright, Appl. Environ. Microbiol. 45:1953-1955 (1983); Vandenbergh, P. A., C. F. Gonzalez, A. M. Wright and B. S. Kunka, Appl. Environ. Microbiol. 46:128-132 (1983); Vandenbergh, P. A., R. H. Olsen and J. F. Colaruotolo, Appl. Environ. Microbiol. 42:737-739 (1981); and Vandenbergh, P. A., and R. L. Cole, Appl. Environ. Microbiol. 52:939-940 (1986). The useful application of bacteria to the environment to degrade organic wastes has been previously demonstrated by U.S. Pat. No. 4,593,003 to Vandenbergh. Also, U.S. Pat. No. 4,910,143 to Vandenbergh describes a mixture of
Pseudomonas putida
strains for degrading an organic waste of the kind that are commonly found in the environment. Also, U.S. Pat. No. 4,673,505 to Wong; 4,822,490 to Dyadechko et al; U.S. Pat. No. 4,956,295 to Sudoma; U.S. Pat. No. 4,970,000 to Eppler et al; U.S. Pat. No. 4,999,301 to Bryan-Jones; U.S. Pat. No. 5,039,415 to Smith; and U.S. Pat. No. 5,185,080 to Boyle show various bacteria and methods for organic waste degradation.
The problem with the prior art methods is that it is too costly for a culture supply house to provide sufficient microbial cells on site for direct inoculation at an effective level into the organic waste compositions. The shipping costs are also significant for such large amounts of culture. The user on the other hand needs as many cells as possible for the degradation of the wastes. There is thus a need for a method which provides this level of microbial cells on site for inoculation into the organic wastes.
OBJECTS
It is therefore an object of the present invention to provide a method for growing specific cells in a disposable, microbiologically prepared (essentially aseptic) container so that the cells multiply by 10 within 24 hours and under acceptable microbial conditions to eliminate the growth of unwanted bacteria cells. It is further an object of the present invention to provide a method which uses multiplied cells for degrading an organic waste, wherein sufficient cells are grown at the site for inoculation of the microorganisms into the waste material, preferably at least about 10
6
cells/ml of the organic wastes. Further still, it is an object of the present invention to provide a method for degrading organic wastes using microorganisms that is relatively inexpensive and easy to use. These and other objects will become increasingly apparent by reference to the following description and to the drawings.
REFERENCES:
patent: 4138036 (1979-02-01), Bond
patent: 4385121 (1983-05-01), Knowlton
patent: 4415085 (1983-11-01), Clarke et al.
patent: 4452894 (1984-06-01), Olsen et al.
patent: 4593003 (1986-06-01), Vandenbergh
patent: 4672037 (1987-06-01), Daggett et al.
patent: 4673505 (1987-06-01), Wong
patent: 4786192 (1988-11-01), Graves et al.
patent: 4822490 (1989-04-01), Dyadechko et al.
patent: 4910143 (1990-03-01), Vandenbergh
patent: 4911832 (1990-03-01), Miller et al.
patent: 4956295 (1990-09-01), Sudoma
patent: 4970000 (1990-11-01), Eppler et al.
patent: 4994391 (1991-02-01), Hoffman
patent: 4999301 (1991-03-01), Bryan-Jones
patent: 5039415 (1991-08-01), Smith
patent: 5185080 (1993-02-01), Boyle
patent: 9002167 (1990-03-01), None
Vandenbergh, P.A. and A.M. Wright, Appl. Environ. Microbiol. 45:1953-1955 (1983).
Vandenbergh, P.A., C.F. Gonzalez, A.M. Wright ) and B. S. Kunka, Appl. Environ. Microbiol. ) 46:128-132 (1983).
Vandenbergh, P.A., R.H. Olsen and J.F. Colaruotolo, Appl.Environ. Microbiol. 42:737-739 (1981).
Vandenbergh, P.A. and R.L. Cole, Appl. Environ. Microbiol. 52:939-940 (1986).
Glendening Larrick H.
Scuilla Vincent J.
Beisner William H.
McLeod Ian C.
Osprey Biotechnics, Inc.
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