Method for using lead-sensitive enzymes to determine levels of l

Details Method for using lead-sensitive enzymes to determine levels of l Method for using lead-sensitive enzymes to determine levels of l

1994-02-22

1997-10-21

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

436 74, 436815, C12Q 134, G01N 3320

Patent

active

056795386

ABSTRACT:
A method is described for determining the level of lead in blood and other types of samples. The level of lead is determined by using a lead-sensitive enzyme. As a first step, a native lead-sensitive enzyme in the sample is disabled. This step may be omitted if the sample is known not to contain any lead-sensitive enzymes. A quantity of lead-sensitive enzyme with known enzymatic activity is then added to the sample. The new enzymatic activity of the sample is then measured. Finally, the level of lead in the sample is determined from the measurement of the new enzymatic activity, which differs from the known enzymatic activity if lead is present in the sample.

REFERENCES:
patent: 3973129 (1976-08-01), Blumberg
Witting U., Evaluation Of A New Specific Analysis . . . Int Arch Occup Environ Health 1987 59:375-383.
Verebey R., Rapid Sensitive Micro Blood Lead Analysis . . . J Of Anal Toxicology vol. 15 Sep./Oct. 1991, pp. 237-240.

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