Method for using hydratase or a hydratase-amidase fusion for ste

Details Method for using hydratase or a hydratase-amidase fusion for ste Method for using hydratase or a hydratase-amidase fusion for ste

1998-06-24

1999-03-30

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing nitrogen-containing organic compound

435 41, 435136, 435228, 435232, 4352523, 435877, C12P 1302, C12P 740, C12N 980, C12N 988

Patent

active

058887857

ABSTRACT:
The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitrites to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.

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